Catalog data:  This course is designed to accompany Biochemistry I.  It will provide an introduction to laboratory techniques used in the isolation and characterizatio of the major classes of biological molecules.  Emphasis will be placed on techniuqes for protein urification and assay including chromatograph, electrophorestic methods, centrifugation, spectroscopy, and enzyme kinetics.  Offered in the Fall semester.  One semester; onoe credit.

Prerequisites:  BIOL 112, 112L, CHEM 212, 212L.  Prerequisite or Corequisite: CHEM 315.

Textbooks:   Biochemistry Laboratory: Modern Theory and Techniques, by Rodney Boyer, Benjamin Cummings, 2006; ISBN: 9780805346138 .

Calculator Policy:   Scientific Calculator

Goals:  The course is designed to:
1) Introduce students to the basic techniques and procedures used in the biochemistry laboratory including: spectrophotometry, chromatography, centrifugation, and enzyme kinetics;
2) Illustrate principles discussed in Chemistry 315 .

Experiments:
pH and Buffers
Paper Chromatography/Identification of Unknown Amino Acids
Gel Filtration: Separation of BSA from Phenol Red
Protein Isolation
Introduction to Spectrophotometry/Protein Determinations
SDS Electrophoresis/Molecular Weight Determination
Enzyme Kinetics
Separation of Proteolytic Digests
Isoelectric Focussing
Electrophoresis and Identification of Phosphoproteins
Inversion of Sucrose
Measurement of Cholesterol and Vitamin C in Biological Samples
DNA Fingerprinting
Bacterial Transformation
Isolation and Characterization of DNA

Grading: 
6 laboratory reports @ 100 points each 600 points
Homework 100 points
Mid-term Examination 75 points
Comprehensive Laboratory Final 150 points

Final Examination: A comprehensive final examination

Attendance: Regular laboratory attendance is required.

Resources:  CBU Internet resources

Coordinator:  Dr. Dennis Merat