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Catalog Data
| This course is a study of the various compounds and reactions considered
in CHEM 312. Typical biochemical analytical methods such as spectrophotometry,
chromatography and centrifugation are utilized. A rigorous study of enzyme
kinetics is included. |
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Textbooks
| Modern Experimental Biochemistry; 2nd ed.; Rodney F. Boyer; Benjamin/Cummings,
California, 94065; ISVN 0-8053-0545-9. |
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Prerequisite:
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CHEM 212 and CHEM 212L |
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Corequisite:
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CHEM 312 |
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Calculator Policy
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Goals
| The purpose is to provide junior-senior students majoring in chemistry
or biology with a modern and complete experience in experimental biochemistry.
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| Syllabus |
Experiments:
Measurement of pH
Buffers
Separation and purification of Biomolecules by Chromatography:
Amino Acid Separation
Characteriztion of proteins and Nucleric Acids by Electrophoresis
Purification and Characterization of a -Lactalbumin
Protein-Lingand Interactions
Vitamin C content from Fruits, Vegetables, and other Foods
Extraction of Chromosomal DNA from Bacterial Cells |
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Final Exam
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Resources
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Attendance
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Coordinator
| Dr. Marguerite B. Cooper |
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Computer Useage
| HP8453 Diode-Array Spectrophotometers. |