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Biol
217 Human Anatomy and Physiology I
Lab
#1 (Anatomical Terminology and Introduction to Lab Resources)
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Torso Models
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Computer Resources
(in AH107)
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Required:
Log
on to WebCT
and take the practice quiz!
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Netter's Interactive Atlas (Installed
on AH107 computers)
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ADAM Practice Practical (Installed
on AH107 computers)
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Dynamic Human CD
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Practice tests, etc.
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Full size PowerPoint
Lecture slides are available on \\facstaff\biology.
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Web Sites for lab
#1 and text Ch. 1
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Chemicals of Life
(text Ch. 2): Web Sites
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Lab
#2 (Microscopy, Cell Structure, Mitosis)
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Web Assignments: Inner
Life of a Cell (versio
with no narration, music)
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Dynamic Human (CD) Assignment

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Human Body: Anatomy
(cell
components, shape, size)
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Explorations: Cell cycle
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Videotape/DVD:
Histology
Video Series, Vol 1 Cells
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Digital images for Lab
#2 are available on \\facstaff\biology:
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Cytology: Demonstrating
staining of various cell components; refer to annotated list in Supplement.
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Mitosis
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PowerPoint lecture
slides are available on \\facstaff\biology
and WebCT.
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Lab
#3 (Osmosis, pH, Buffers)
Diffusion
and Active Transport Definitions (modified
from Dr. James
S. Miller)
1.
Simple
diffusion - transport through the lipid phase of the membrane; rate
dependent on lipid solubility and concentration gradient; does not require
ATP, passive.
2.
Facilitated
diffusion - transport through a protein carrier/pore; rate dependent
on concentration gradient and carrier/pore efficiency (and of course also
the number of proteins carriers); does not require ATP, passive.
(Some authors use "facilitated diffusion" for carrier mediated transport
but not for channel mediated transport, as for ions. However, most
prefer to include both under "facilitated" diffusion".) Note that
the rate of facilitated diffusion can be no faster than that of simple
diffusion.
3.
Active
transport - transport via a protein that is linked to energy use;
transportation can be against a concentration gradient and can create a
concentration gradient
1 &
2 (simple diffusion and facilitated diffusion) can both be referred to
as "passive transport" - no energy consumption is directly involved
2
& 3 (facilitated diffusion and active transport) can both be referred
to as "carrier mediated" - a carrier protein/pore is involved, and transport
exhibits properties of saturation and competition |
 
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Cell Metabolism:
Text Ch. 4 PowerPoint Lecture slides are available
on \\facstaff\biology and WebCT.
Q: Why are
fats the primary stores of energy, as opposed to carbohydrates?
A: The main advantage
is that fats are a lower density energy supply; 5 grams of fat contain
the same amount of bond energy as 9 grams of carbohydrates. Fat molecules
also don't have the hydration shell that surrounds carbohydrates. -- Ruth
Buskirk, University of Texas NABT
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"...gram for gram, fats
provide more energy than carbohydrates."
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"When you weigh a carbohydrate,
more oxygen is included in that weight. When you weigh a fat, you get more
carbon atoms per gram and therefore, gram for gram, the fats will give
even more energy (over twice as much) than will the carbohydrates. Generally,
fats provide about 9 kilocalories per gram and carbohydrates provide about
4 kilocalories per gram. (Using nutritional units, that is 9 Calories/gram
for fats and 4 Calories/gram for carbohydrates.)"
Source: http://dl.clackamas.edu/ch106-07/carbohyd1.htm
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Goblet cell, microvilli
Compact Bone
Hyaline Cartilage
Integument
Links
to
Histology
Sites.
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Lab
#4 Tissues and Skin.
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1. The microscope slides and CD's used in
Lab #4 (Tissues and Skin) are available for your use during study
lab times (i.e., any time 8:00 am--5:00 pm when there is not another
class in AH107). Lecture slides are available on \\facstaff\biology
and on WebCT.
I can also make a CD of lab images for you.
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You can use the digital images on \\facstaff\biology
from any computer on campus. Use ACDSee
to view the images.
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Study Hole Ch. 5 and 6 plus Benson Exercises
10, 11, & 12. Also, work on the Hole Ch. 5&6 worksheets and read
the Lab #4 material in the Supplement.
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2. Web Sites
assigned for Lab #4:
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3. Lab #4 List of Microscope Slides (examples
to know, etc.). See Supplement.
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4.Videotapes/DVD's
on tissues and skin:
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Histology
Video Tape Series (DVD & VHS): Vol. 3 Epithelial Tissues, Vol.
4 Connective Tissue, Vol. 6 Cartilage, Vol. 7 Bone, and Vol. 10 Skin.
(Each tape is ~30 min. long and includes a practice practical at the end.)
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5.
PowerPoint
Lecture slides are on \\facstaff\biology.
and on WebCT
Study the following
PowerPoint Slides (the photomicrographs will help you prepare
for the lab quiz, lab midterm exam, and Lecture Exam 2)
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Ch. 5 part 1 Simple Epithelia
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Ch 5 part 2 Stratified
Epithelia and Glandular Epithelium
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Ch 5 part 3 Connective Tissue
Proper
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Ch 5 part 4 Special C.T., Muscle,
and Nervous Tissue
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Ch 6 Integumentary System
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5. What's on the
Lab quiz?
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Digital images where I'll
ask "Name the Tissue" and/or "Identify
the Source" [3-4 points] (Know the examples listed in the
Supplement.)
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Short answer questions where
I'll name the location and you name the tissue
and/or I name the tissue and you name one or more locations
where it is found. [approx. 1/2 the quiz] (Know the examples
listed in the Supplement.)
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Explain, define and use terms
for classifying tissues (I'll ask about one or more specific tissue
examples and/or terms) [Approx. 3 points]
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Don't worry that I've "left
out" some of the details on skin: (nearly) ALL the anatomical wonders of
skin WILL be included on the Lab Midterm!
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Labs
#5 and #6 (Skeleton and Joints).
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Benson Exercises 13, 14, 15, 16, & 17.
Hole Chapters 7 & 8.
Supplements (Yokochi, ADAM
Atlas, Van De Graaff).
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Lab #5 deals with
the skeletal system, especially the skull. (Yes,
ALL the little holes, nooks, crannies, and things you never realized actually
HAVE names.)
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Quiz # 5 will cover
the skull
(ID
bones, parts of bones, sutures, and foramina from diagrams, photos, and/or
real skulls or model skulls).
Sex Characteristics of the Skull
|
| Feature |
Male |
Female |
| A. supraorbital ridge |
prominent |
slender |
| B. occipital protuberance |
prominent |
slender |
| C. mastoid process |
long, broad |
short |
| D. mandible |
square |
V-shaped |
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The study of the
skeletal system and joints continues in Lab #6.
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See Supplement and the PowerPoint slides
for Hole Chapters 7 and 8.
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Materials available
for study in AH107:
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Human bones, medical-grade plastic casts of
human bones (please handle with care; use only the
designated "safe" pointers and tools)
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Dissectable skull; Disarticulated skull bones
(please
keep each bone in it's labeled plastic bag)
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Fetal skull and medical-grade plastic cast
of fetal skull
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X-rays
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Models of knee joint
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Videotapes/DVDs:
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Wm. C. Brown Skeletal System
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Anatomy dissection Tape #4: Head.
Skull bones Shows excellent detail. (VHS & DVD)
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Height Estimation Using
the Femur
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| Male: (2.32 x length
of the femur in cm.) +65.53 ± 3.94 |
| Female: (2.47 x length of
the femur in cm.) + 54.10 ± 3.72 |
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Computer Resources
in AH107:
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Atlas Plus:
Advanced
Tools
for Learning
Anatomical
Structure.
CD
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Anatlab:
The
Anatomy Lab. Human anatomy laboratory and tutorial. CD
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Human
Anatomy Online (Gold Standard) Dissection
of Human Cadaver
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Radiologic Anatomy
CD (Gold Standard) ID structures in X-Ray - Tutorial
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Netter's Interactive
Atlas (installed on the AH107 computers)
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ADAM Practice Practical
(installed
on the AH107 computers, PC's)
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A.D.A.M. (Comprehensive installed
and Standard with CD)
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Links for Labs #5
& #6: Skull, Skeletal System and Joints (more
links in Supplement & PowerPoint Slides!)
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The
Skeleton Dance (Disney,
1929) :-)
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Skeleton
tutorials: Skull, vertebrae, and hand. http://www.gwc.maricopa.edu/home_pages/crimando/jctuts5.htm
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Skull
Module: Rotate photos of skull and individual
skull bones.
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Skull
Anatomy Tutorial http://anatome.ncl.ac.uk/tutorials/skulleasy/text/index.html
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Skeletal
System Web Anatomy Tutorial
Quiz
yourself on diagrams of bones and knee joint (including skull)
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Skeletal
System Interactive with photos (Penn State)
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Skeletal
Interactive Quizzes with bone photos (NHC)
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Interactive
Osteology
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"Bone
Box" at LUMEN Color photos of bones.
Point and click to name bones and their major features.
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Lessons
from a bone box (U. Iowa) QT videos
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Human
Anatomy Dissector online (cadaver photos)
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Human
Skeletal System Interactive Review from Univ. Minn.
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Appendicular
Skeleton: Vocabulary and Practice Test.
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Bones
Practice Test
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Gender
Guide: Skull
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Craniometrics
to identify ethnic origin
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Gender
Guide: Pelvis
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Living
Skeleton: Labeled and unlabeled Xray images
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Normal
Knee Anatomy (Univ. of Washington teaching
modules)

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Knee
Anatomy Tutorial http://anatome.ncl.ac.uk/tutorials/knee/text/index.html
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Radiographic
Anatomy of the Skeleton (Univ. of Washington)
X-rays. Click to see labeled images.
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Osteoporosis
and Bone Physiology (Dr. Ott)
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Osteoporosis
tutorial from U. Utah
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Osteoporosis
(National Osteoporosis Foundation)
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Assess
Your Risk of Osteoporosis
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Parathyroid.com
includes info on normal Calcium metabolism
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Normal
Radiologic Anatomy archive from U Iowa X-rays,
MRI's, etc. Includes a self-quiz section.
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Joint
Fluoroscopy archive from U Iowa - View
the section on the Knee joint for Lab #6 material.
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Knee
Anatomy (Interactive Atlas from Digital Anatomist). Color
coded 3-D images. Click on an image, then ask for labels, quiz,
etc. with buttons at the bottom of the screen.
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Virtual
Knee Surgery http://www.edheads.org/activities/knee/index.htm
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ACL
injuries in female athletes
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Smith
& Nephew presents Interactive Hip, Knee, and Shoulder (cool
flash animations with labels)
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Your
Orthopaedic Connection: Educational informa tion from Amer. Academy
of Orthopaedic Surgeons (patient info on joint replacement, fractures,
etc.)
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Hole's
textbook website ("Online Learning Center" with practice
quizzes, etc.)
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Practice
tests (try the matching exercises to practice labeling images)
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PowerPoint
Lecture slides are available on \\facstaff\biology
and on WebCT
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Study
the following Lecture PowerPoint Slides:
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Chapter 7 part 1 Bone histology
and physiology
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Chapter 7 part 2 Bone identification
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Chapter 8 Joints (Lab
exam emphasis is on the Knee Joint)
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This material is included
on the Lab Midterm and Lecture Exam 3.
 
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Just for fun: http://www.chezmaya.com/applet/valentin.htm
Addictive... the skeleton
responds to your cursor ... notice, however, that this "puppet" is impossibly
limber.
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Links
to
A&P
Sites.

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Lab
7: Midterm Lab Exam & Begin Study of Cat Muscles
BIOL
217 A&P Lab Midterm Exam
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Covers all topics from the first six lab sessions.
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Includes handouts, Benson lab manual, Supplement,
digital images, and textbook/lecture slide information on the lab
topics.
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100 points (1/3 of the lab course grade)
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Topics
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Anatomical
terms, membranes, body cavities [~12 pts.]
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Microscopy,
cell structure, mitosis [~10 pts.]
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Osmosis,
pH, buffers [~14 pts.] ** Review this!
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Tissues
[~16 pts.]
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Integument
[~10 pts.]
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Skull
[~16 pts.]
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Skeleton,
joints (knee joint) [~26 pts.]
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Format:
Mostly practical; All short answer (ID the structure, etc.)
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Diagrams
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Anatomical Models
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Microscope slides
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Digital images (from \\facstaff\biology
and lecture slides)
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Skull (adult and fetal)
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Bones (individual)
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Written questions (short answer/objective)
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Labs
7, 8, & 9: Muscle Anatomy
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Benson Exercises 19, 33, 34, 35, 36, 37, 38,
39, and 40.
Assigned homework includes
the Benson lab reports. (Complete Ex. 19, 33, 34, 35,
36, & 37 for Lab 8. Complete Ex. 38, 39, & 40 for Lab 9.)
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Hole Chapter 9.
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Supplements (Yokochi, ADAM Atlas, Van De Graaff).
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Web Sites (more
are listed below):
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Materials
for Labs 7, 8, and 9: (Handouts &/or in
Supplement)
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1. Worksheet
for Hole Chapter 9: Muscular System. [Also for Lab #10-Muscle
Physiology]
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2. Human
Muscles Lab Worksheet (diagrams to label)
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3. Human
Musculature Video (list of muscles identified on the video)
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4. [2 Sides] Muscles of the
Upper Extremity/Muscles of the Lower Extremity (Worksheet
to complete using the models.)
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Quiz # 6: CAT
MUSCLES:
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Superficial
muscles of the neck, chest, trunk, and shoulder. The quiz will require
recall memory. (Name the muscles indicated on diagrams or pinned on cats.)
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Quiz # 7: CAT
MUSCLES:
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Superficial
muscles of the forelimb; superficial and deep muscles of the abdomen, hip,
and hindlimb. The quiz will require recall memory. (Name the muscles
indicated on diagrams or pinned on cats.)
Lab
7: Cat Muscles
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The study of muscles begins in Lab #7,
following the Lab Midterm Exam. You will work with a team using a
preserved cat to expose, examine, and identify cat muscles.
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Correction to
the Benson Lab Manual: fix the label for the gracilis
muscle (vs. sartorius) on the photo of the medial thigh (page 131).
Lab
8: Cat Muscles and Human Muscles
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Lab #8 covers the anatomy of the skeletal
muscles, especially the cat. (You will work with a team to
dissect the cat exposing muscles of the chest, back, shoulder and upper
limb, hip and lower limb.)
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We will study all the required cat muscles
during Labs #7-8. Cat muscles are covered on
lab Quiz # 6.
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During Lab #8, teams will begin using the
computer resources to study human muscles (so that each team will be able
to use all the resources by the end of Lab #9).
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Videotape/DVD: Cat Muscles (1 hr.) Videos/DVDs
are also available in AH107 during study labs.
Lab
9: Human Muscles
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Lab #9 covers the identification of
human skeletal muscles. During lab you will work with a team
to use the computer resources, video tapes, and models. You will
complete several worksheets.
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Benson lab reports.
Complete
Ex. 19, 33, 34, 35, 36, 37, 38, 39, & 40.
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Lab Quiz # 7 covers human muscles.
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Videotape Assignments
(Human
Muscles):
Students may also use
the videos in AH107 during Study Lab times.
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Human Muscles (Benjamin/Cummings
25 min.). Fill in the worksheet.
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Human Muscles (1
hr.)
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Cadaver
Atlas video series. (DVD & VHS)
Muscles
of the upper extremity, Muscles of the lower extremity, Muscles of trunk,
etc.
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Computer Assignments:
(CD's etc.)
Students may also use
these materials in AH107 during Study Lab times.
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Interactive Physiology
CD: Muscular System [Also
for Lecture Exam #3 preparation and Lab #10-Muscle Physiology]

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Human
Muscle animations (Get Body Smart)
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Human
Anatomy online (Gold Standard) Dissection
of Human Cadaver
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Netter's
Interactive Atlas (Installed on AH107 Computers)
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ADAM
Practice Practical (Installed on AH107 PC's)
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Atlas
Plus: Advanced Tools
for Learning Anatomical
Structure.
CD
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Anatlab:
The Anatomy Lab. Human anatomy laboratory and tutorial. CD
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Harper Collins Physiology
Animations: Module III Events at the Neuromuscular Junction
[Also for Lab #10-Muscle Physiology] (Installed on AH107 Computers)
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Web sites: Muscle
Review: Vocabulary and practice questions.
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Exercise
and human muscle directory http://www.exrx.net/Lists/Directory.html
Excellent illustrations and video clips of esercises.
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Just for fun: http://www.chezmaya.com/applet/valentin.htm
Addictive... the skeletom responds to your cursor ... notice, however,
that this"puppet" is impossibly limber.
| Human Hamstring Muscles: Here's
a suggestion on how to remember the relative positions of these three muscles...
The "Semi's" go together: Semitendinosus
has a long tendon and Semimembranosus
is more medial. The Biceps
femoris is "by" itself on the lateral aspect
of the thigh. (Source: Krieger, Paul.
20004. Using creative analogies to teach A&P. HAPS Educator,
Fall 2004: p. 27.) |
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Web
Resources: Human Muscle Anatomy
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Note: Lecture Exam #3
includes identification of human muscles, including a diagram to label.
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The Final Lab Exam will include HUMAN
Muscles, but no cat muscles. You will need to be able to recognize human
muscles on models and/or diagrams. You will need to know the NAMES and
ACTIONS of the human muscles we study.
PowerPoint lecture
slides are available on \\facstaff\biology
and on WebCT.
"Rigor
mortis seems to be due to the final absolute
depletion of ATP in the muscles, which then stops the cycle of actin-myosin
activity at the point at which new ATP would be used: namely, the
detachment of myosin from actin. This leaves all the myosin crossbridges
in a permanent state of attachment to actin, with no possibility of relative
movement between any of the thick and thin filaments. With all the
sliding filaments 'frozen up,' the muscle becomes quite rigid. The
subsequent loss of Rigor Mortis is then due to the breakdown of the muscle
tissue that follows." Dr. Steven N. Trautwein
Also see: Medico-legal
problems of establishing the time of death:
... "corpses can usually
be divided into those, still warm, in which no rigor is present, indicating
death within about the previous three hours. Those in which rigor is progressing,
where death probably occurred between 2 and 9 hours previously; and those
in which rigor is fully established, showing that death took place more
than 9 hours previously." "If full rigor is present, then one might assume
that this is about the second day following death, depending upon the environmental
conditions." |
 
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Lab
10: Muscle Physiology and Physiogrip
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Ex 25, 26, 27,
28, and 30.
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BIOPAC
Lesson 1: Muscle contraction, EMG.
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Ex
25 Stimulator Function: Read.
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Ex 26 Intelitool:
Read. This is the Physiogrip system.
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Ex
27: Read (relate to lecture material).
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Ex
28 Mapping motor points:
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Ex 30 Physiogrip:
Do this lab. Lab Quiz #8 will cover Physiogrip
labs 1, 2, and 3. [See Supplement pages 173-176, and PowerPoint
slides for Hole Ch. 9.]
In preparation
for Lab Quiz #8: Study the relevant topics from
your text (Hole Ch. 9) and the PowerPoint slides on \\facstaff\biology,
WebCT, and in your Course Supplement.
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Lab
Quiz #8 will emphasize Physiogrip labs 1, 2, and 3.
You
will need to be able to explain the concepts and draw sample graphs.
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Web Resources on
muscle physiology
-
Computer Resources
on muscle physiology
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Interactive
Physiology CD: Muscular System
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Harper Collins Physiology
Animations: Module III Events at the Neuromuscular Junction
(Installed on AH107 PC's)
| Lactic Acid Helps Muscles
[From Science Roundup by AAAS]
"We've all felt it at some point -- the ache and burn
of muscle fatigue after a long run or intense workout. Conventional wisdom
holds that lactic acid -- generated when physical exertion deprives our
muscles of oxygen and they switch from aerobic to anaerobic means to create
energy -- is to blame for the pain. Now, a report in the 20 Aug 2004 Science
shows that, on the contrary, accumulation of lactic acid actually helps
to maintain muscle function. Using a preparation of skinned rat skeletal
muscle fibers, Pedersen
et al. demonstrated that the increased acidity associated with lactic
acid production decreases the activity of chloride ion channels and helps
muscles maintain their electrical excitability and ability to contract.
These chloride channels normally help maintain the balance of electrical
signals (which also involves sodium and potassium ions) that prevents spontaneous
contractions in rested muscles. An accompanying Perspective by D.
Allen and H. Westerblad highlighted the report and reviewed the history
of lactic acid in muscle fatigue research." |
 
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Lab
11: Nervous Tissue and Nerve Function
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Benson Exercises: 18,
20, 41, and 42 (including handout) + BIOPAC Lesson
11.
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Videos: Histology Video
Series, vol. 9: Nervous Tissue (DVD); Brain and Nervous System
(Spektrum Videothek)
-
Essential references:
Hole, Nervous System chapters and powerpoint lecture slides.
Neural Tissue:
-
Digital images on facstaff\biology
(Carolina
Slide Sets plus other images):
Neurons, Neuroglia, Spinal
cord, Spinal ganglion, Cerebrum, Cerebellum.
-
PowerPoint Slides sets: Nervous System Histology
-
CD-ROMs: Microscopic Anatomy,
etc.
Web
sites
Microscope Slides
(slide
boxes H-# listed in Supplement )
Models:
Spinal cord, dorsal root ganglion and spinal nerve. Neuron, synapse, etc.
Torso model.
Computer Resources
-
Interactive Physiology (CD's): Nervous
System and Nervous System II
-
A.D.A.M. Interactive Anatomy
(avail. on AH107 computers)
-
A.D.A.M. Practice Practical
(avail. on AH107 computers)
-
Netter's Interactive Atlas (avail.
on AH107 computers)
-
Histology CD's
Reading:
Cranial
Nerve Assessment (Handout, from the journal, RN).
Reflex experiments
and Reaction Time (as listed in Supplement; also
see below)
Benson Exercise 42
Reflexes in Medical Diagnosis (start at page 203)
Patellar
Reflex demonstration
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