Homogenization of Gills

• Dissected (gills) from 18 shrimps.

• Homogenized in TRIS-HCL, pH 7.6, buffer in PMSF containing 1mM EDTA, 1mM DTT, 0.5M sucrose, and 0.15M KCL.

• Centrifuged at 18.000 xg for 20 minutes at 4 °C.

• Stored in –80 ºC freezer.

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