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Immunology Lab
Fall 2002
Instructor: Dr. Mary Ogilvie
Email: mogilvie@cbu.edu
Phone: 321-3437
Book: Immunology Lab Protocols 2002, Ogilvie, M. L.
Journal Article:
A typed report in the form of a journal article will be due
Nov. 14. Experiments listed below with a "+"
will be included in the article. I will return the article to
you with suggested changes to be incorporated into the paper for
an improved grade (dueNov. 27). Further instructions for this
article are found in this manual. Homework:
Homework assignments are due the Tues. after Immuno. Lab.
Include experimental results (in table form, if possible) and
interpret the results.
Grading: A = 90-100
2 Exams = 100 pts each B = 80-89
Paper = 100 pts C = 70-79
Homework = ~120 pts D = 60-69
Total = ~420 Pts F = Below 60
| DATE | EXPERIMENTS | CHAPTER |
| 8/22 | Use of micropipetters, Make
PBS +Preparation of immunogens |
1 |
| 8/29 | +Obtain Preimmune Serum from
Rabbits +Immunization of Rabbits |
2 |
| 9/5 | Manipulation of Bacteria +Salt-precipitation of Preimmune Sera 3 Film |
4 |
| 9/12 | Examine Bacterial Plates --
Homework #1 Innate Immunity Wright's Staining of Human Blood Cells-- Homework #2 |
4 5 X |
| 9/19 | +Ouchterlonies (In journal
article, use only preimm. results.) X Summarize Innate Immunity Assays -- Homework #3 +Cellulose Acetate Electrophoresis -- Homework #4 X (In journal article, use only preimm. results.) |
X X |
| 9/26 | +Prepare Booster (I'll boost rabbits
on Fri.) +Review OuchterloniesHomework #5 HCG agglutination Detect-a-Strep assay |
X X X X |
| 10/3 | 10/3 Immunofluorescence.Homework
#6 Exam Review |
X |
| 10/10 | EXAM #1 +Obtain immune rabbit blood at U of M |
|
| 10/17 | FALL BREAK | |
| 10/24 | +Salt precipit. of immune serum +Coat ELISAs with Ag +Make Ab dilutions for ELISA +Dialyze sera for DEAE columns (need in 2 wks) |
X X |
| 10/31 | +Ouchterlonies with immune
sera -(Immune and Preimm. Sera) +Run ELISA assaysHomework #7 |
X X |
| 11/7 | Examine Ouchterlonies. Homework
#8 Do gel samples for Western blots Pour and run DEAE Sepharose Columns Pervaporate samples before next Thurs. |
X X X X |
| 11/14 | Journal Article Due (Rough
Draft) Start Western Blots and Dot blots (thru 1st Ab) XX Cellulose Acetate electrophoresis with Immune serum X |
X X |
| 11/21 | Finish Western BlotsHomework #9
X Finish Dot Blots.Homework #10 X Movie: "And The Band Played On" or "Osmosis Jones" |
X X |
| 11/27 | Journal Article Due (FinalDraft) | |
| 11/28 | Thanksgiving | |
| 12/5 | Final Exam |
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